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Media supplements
| Small-molecule inhibitors | Concentration used | Manufacturer | Product number | Note |
|---|---|---|---|---|
| IWR-1 | 1 µM | Sigma-Aldrich | I0161-5MG | |
| CHIR99021 | 3 µM | Tocris | 4423 | |
| Recombinant proteins | ||||
| FGF9 | 50 ng/ml | Sigma-Aldrich | SRP3040 | |
| Activin A | 25 ng/ml | R&D Systems | 338-AC-010 | |
| Activin B | 25 ng/ml | R&D Systems | 659-AB-005 | |
| TGFβ1 | 25 ng/ml | R&D Systems | 240-B-002 | |
| RSPO1 | 100 ng/ml | R&D Systems | 4645-RS-025 | |
| WNT4 | 100 ng/ml | R&D Systems | 6076-WN-005 | Product discontinued |
