Table 1.

Estimated costs per experiment of 5,000 cells

	10x v2	10x multiome	Bio-Rad ddSEQ	s3-ATAC	HyDrop
Sequenced reads per cell at saturation	55,000	68,000	19,000	1,467,000	10,000
Expected unique fragments per cell	22,427	10,155	5,249	66,130	1,884
Expected unique fragments in peaks per cell	13,680	6,398	2,992	12,565	716
Assay price per 5,000 cells	$1,565	$2,843	$1,100	$800	$100
Sequencing cost	$791	$978	$273	$21,088	$144
Total cost per cell	$0.471	$0.764	$0.275	$3.80	$0.049

Saturation sequencing depth is defined as the depth at which 50% of fragments in cells are duplicates. The expected number of unique fragments was calculated as the expected number of unique fragments at saturation sequencing depth, interpolated or extrapolated using a Langmuir model, and multiplied by the median FRIP score per technique to achieve the expected in peaks count. The price per 5,000 cells is for one 10x lane, one ddSEQ lane, four s3-ATAC plates (1,440 cells each, for 5,760 cells in total) or one HyDrop ATAC run. Sequencing cost is defined as the price of sequencing 5,000 cells to saturation depth, calculated at a cost of $2.875 per 1 million reads, using NovaSeq S2 100 cycles (10x v2, 10x multiome and HyDrop) or 200 cycles (ddSEQ and s3-ATAC; 4,000 million reads sequenced at $10,000 to $11,000 total cost). The sequencing cost for 10x multiome RNA component was not included.