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. 2024 Jun 3;15:1397656. doi: 10.3389/fphar.2024.1397656

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Copyright © 2024 Lu, Shi, Cheng, Wang, Xu, Sun, Zhang, Yang, Li, Wu and Kuai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative immunoreactivity patterns of TILs and immune checkpoint expression in gastric cancer tissues: (A) GC immune environment using multiplex fluorescence IHC (mIHC) panels scanned using Vectra software. A1: lymphocyte panel of CD3, CD4, and CD8 with CK staining. A2: B lymphocyte panel of CD20 with CK staining. A3: myeloid panel of CD66B and CD68 with CK staining. A4: immune therapy target panel of PD-1 and LAG3 with CK staining (green: CK; blue: DAPI; left column magnification ×20; right column magnification ×400). (B) Quantified protein expression using the immunohistochemistry (IHC) score (intensity: brown, intense staining; orange, moderate staining; yellow, weak staining; and blue, no staining. Left column magnification ×20; right column magnification ×400). B1: CTLA4 in cancer tissues. B2: CTLA4 expression in cancer cells was scored using software. B3: CTLA4 expression in TILs was scored using software. B4: PD-L1 in cancer tissues. B5: PD-L1 expression in cancer cells was scored using software. B6: PD-L1 expression in TILs was scored using software.