Figure 1.

Deletion of GR specifically in BMAds using the BMAd-Cre mouse model. (A) Breeding strategy for generation of BMAd-Nr3c1 ^-/- mice. (B) PCR amplification was used to detect the presence of loxP sites flanking exon 3 of Nr3c1 (top panel, primers Fwd + R1). PCR products demonstrate presence of a band lacking exon 3 (bottom panel, primers Fwd + R2) in tail and bone marrow (BM) of BMAd-Nr3c1 ^-/- (KO) mice but not in subcutaneous WAT (sWAT), epididymal (eWAT), or liver. Recombination was not detected in BMAd-Nr3c1 ^+/+ mice (WT).