Figure 2.

Female BMAd-Nr3c1 ^-/- mice have slightly elevated tibial bone variables. (A) Female mice at 31–39 weeks of age were euthanized. Tibiae, tail, and femurs were decalcified, embedded, paraffin-sectioned, and stained with H&E. Representative photos are shown. Scale bar: 0.22 mm. (B–D) A schematic of the tibia illustrating locations of µCT slices. Tibiae were analyzed by µCT for (B) proximal trabecular metaphyseal, (C) mid-cortical, and (D) distal cortical bone variables. Tb: Trabecular, BV: Bone Volume, TV: Total Volume, Conn. Dens.: Connective Density, M. Ct.: Mid-Cortical, Dist., Ct.: Distal Cortical. Statistical analysis was performed using an unpaired t-test. *p<0.05.