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. 2024 Jun 3;15:1397081. doi: 10.3389/fendo.2024.1397081

Figure 3.

Figure 3

CR leads to decreased bone length in developing male mice independent of GR in BMAd. Male mice (10-16 weeks old) were fed ad libitum (NCD) or placed on a 30% CR diet for 6 weeks. (A) B ody weights of mice were measured throughout the experiment. Statistical analysis was performed using a two-way ANOVA. *p<0.05 compared to BMAd-Nr3c1+/+ NCD . (B) At the time of euthanasia, tissue weights of sWAT, eWAT, and liver were measured. (C, D) Following necropsy, the length of the tibia (C) and femur (D) were measured. Tibiae were analyzed by μCT for (E) proximal trabecular metaphyseal, (F) mid-cortical, and (G) distal cortical bone variables. Tb: Trabecular, BV: Bone Volume, TV: Total Volume, Conn. Dens.: Connective Density, M. Ct.: Mid-Cortical, Dist. Ct.: Distal Cortical. Statistical analysis was performed using a one-way ANOVA with a Tukey multiple comparison post hoc test. *p<0.05.