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Microfabricated migration assays. (A) Microchannels with MDA-MB-231 Lifeact-GFP/H2B-RFP migrating inside; (B) 5 µm in deep groves with HeLa Tubulin-GFP/H2B-RFP on top, oriented according to the topography (some cells go at the bottom of the groves, see center right, while others stay on the top of it, see left); (C) pillars 7 µm high with HeLa Tubulin-GFP/H2B-RFP plated on top.
