Figure 8.

In-vitro inhibition of NET formation by tacrolimus: Isolated neutrophils from healthy pigs (n = 3) were stimulated using ionomycin to induce NET formation in the presence of different concentrations of TAC (0, 25, 50 and 100 ng/ml). (A) NETs were identified using DAPI (blue) and imaged through confocal microscopy. White arrowheads indicate extruded DNA content from neutrophils, confirming the presence of NETs. (B) NET percentages were calculated as the number of NETs/total number of neutrophils per field, using 5 representative images per condition. ****p<0.0001 by two-way ANOVA with Tukey’s multiple-comparison test.