Fig. 6. Purified yields and enzymatic activity of the GLuc expressed in B strains using SHuffle and CyDisCo from Ptac promoter in rich and chemically defined autoinduction media.

(A) The calculated yields of Gluc expressed in SHuffle express and BL21(DE3) + CyDisCo. (B) Measured luminescence activity per microgram of purified Gluc protein. Note Gluc was not produced using SHuffle in defined media and therefore the activity could not be tested. Bars are mean + SD (n = 3). p ≤ 0.01 (**); p ≤ 0.001 (***).