TABLE 3.
QC-RT-PCR quantification of tissue-specific EIAV singly spliced mRNA in experimentally infected equids
| Tissue samplea | Mean copies of viral singly spliced mRNA/μg of total RNAb
|
|||||
|---|---|---|---|---|---|---|
| EIAVWyo, acute, in animal:
|
EIAVPV, acute, in animal:
|
EIAVPV, asymptomatic, in animal:
|
||||
| 298 | 94-07 | 112 | 123 | 18 | 72 | |
| Brain | ND | 7,000 | <30 | 100 | ND | ND |
| Kidney | 650 | 27,000 | <30 | 650 | <30 | <30 |
| PBMC | 700 | 6,000 | <30 | 17,000 | <30 | 190 |
| Bone marrow | 1,500 | 6,000 | 130 | 700 | <30 | <30 |
| Lymph node | 130,000 | 90,000 | 90 | 1,600 | <30 | 30 |
| Liver | 110,000 | 76,000 | 3,600 | 23,000 | 740 | 140 |
| Spleen | 7,300,000 | 92,000 | 185,000 | 510,000 | 50 | 750 |
a
The brain and the kidney are as described in Table 2.
b
ND, not determined. The values represent the mean copies of viral singly spliced mRNA per μg of total RNA. All data represent the results of at least two independent assays. The standard error was <25%.