FIGURE 3.

ADP-interactions of Myo1B^F387Y and Myo1E^Y388F in the absence of actin. (A) Single fluorescence transients as monitored upon mixing 1 µM Myo1B^F387Y pre-incubated with 0, 2 or 10 µM ADP with excess ATP. (B) Normalized amplitudes of the slow phase (open circles) and fast phase (filled circles) as obtained from biexponential fits to the transients observed upon mixing 1 µM Myo1B^F387Y pre-incubated with increasing concentrations ADP with excess ATP. (C) Single fluorescence transients as monitored upon mixing 1 µM Myo1E^Y388F pre-incubated with 0, 40 or 125 µM ADP with excess ATP. (D) Normalized amplitudes of the slow phase (open circles) and fast phase (filled circles) as obtained from biexponential fits to the transients observed upon mixing 1 µM Myo1E^Y388F pre-incubated with increasing concentrations ADP with excess ATP.