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. 2024 Jun 3;15:1393952. doi: 10.3389/fphys.2024.1393952

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Copyright © 2024 Diensthuber, Hartmann, Kathmann, Franz and Tsiavaliaris.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of [Mg²⁺]_free on ADP release from actomyosin. Relative mantADP-fluorescence time traces as observed upon mixing 1 μM A∙Myo1B^F387Y (A) or 1 μM A∙Myo1E^Y388F (C) pre-equilibrated with 80 µM mADP at 0.05 mM and 4.55 mM free Mg²⁺-concentrations, with excess ADP. Rate of ADP release ( k _-AD) from A∙Myo1B^wt and A∙Myo1B^F388Y (B) and related Myo1E constructs (D) as a function of the free Mg²⁺-concentration as obtained from single exponential fits to the mantADP-fluorescence time traces.