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. 2024 Jun 3;15:1393952. doi: 10.3389/fphys.2024.1393952

FIGURE 6.

FIGURE 6

Effect of [Mg2+]free on myosin motor activity and landing rate. (A,C) Average actin sliding velocities of wild-type and mutant myosins at increasing free Mg2+-concentrations. Myo1Bwt and Myo1EY388F display no Mg2+ sensitivity. (B) Landing rate as a function of Myo1BF387Y motor density. (D) Landing rate as a function of Myo1Ewt and Myo1EY388F motor density. The landing assays were performed at 5 mM (filled symbols) and 0.25 mM (open symbols) free Mg2+ concentrations.