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. 2024 Jun 3;15:1393952. doi: 10.3389/fphys.2024.1393952

TABLE 4.

Regulation of myosin motor activity and duty ratio.

Parameter	Myo1B^wt ^a		Myo1B^F387Y ^a		Myo1E^wt ^a		Myo1E^Y388F ^a
In vitromotility assays
v_max (µm s^-1)	1.76 ± 0.11		1.68 ± 0.06		0.73 ± 0.05		0.64 ± 0.06
v_min (µm s^-1)	1.61 ± 0.09		0.82 ± 0.1		0.31 ± 0.05		0.58 ± 0.05
K_i (mM)	n.a.		0.19 ± 0.03		0.13 ± 0.02		n.a.
Hill coefficient (n)	n.a.		1.96 ± 0.17		2.11 ± 0.21		n.a.
Landing assays

	[Mg²⁺]_free		[Mg²⁺]_free		[Mg²⁺]_free		[Mg²⁺]_free
	250 µM	5 mM	250 µM	5 mM	250 µM	5 mM	250 µM	5 mM
Landing rate order	n.a.		7.2 ± 1.5	2 ± 1	2.6 ± 0.8	1.1 ± 0.1	7 ± 1.1	5.1 ± 0.9
Duty ratio (n⁻¹)	n.a.		0.14 ± 0.02	0.5 ± 0.1	0.38 ± 0.11	0.91 ± 0.07	0.14 ± 0.02	0.2 ± 0.04

^{^a}

Data of this study.