Figure 5.

Treatment with cyclosporin (CsA) decreased Hesperetin (Hst)-induced PINK1-driven mitophagy in palmitic acid (PA)-stimulated HepG2 cells. Cells were pretreated with or without CsA (5 μM) for 4 h in the absence or presence of 40 μM Hst before the addition of PA (400 μM). (A) Representative immunoblotting and (B–D) quantification of the indicated proteins. Data are presented as mean ± SEM of n = 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 (unpaired two-tailed student’s t test).