Figure 8.

PINK1-directed mitophagy involved preventive effects of Hesperetin (Hst) in palmitic acid (PA)-treated HepG2 cells. Cells were transduced with control-siRNA or Pink1-siRNA, and then preincubated with or without 40 μM Hst for 4 h prior to exposure to 400 μM of PA. (A) Representative immunoblotting and (B–D) quantification of indicated proteins. Representative immunostained images of (E) Parkin and Tom 20 in HepG2 cells. Nuclei were counterstained with DAPI (blue). Data are presented as mean ± SEM of n = 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 (unpaired two-tailed student’s t test). Scale bars, 10 μm.