FIG. 5.

Analysis of the vector transduction rate and protein profile in cellular extracts and extracellular viruses from cells transfected with 5′ UTR-deleted packaging constructs, an env expression plasmid, and a vector genome. (A) Schematic view of pCgp packaging and pMH104 vector constructs and vector transduction rate on HT1080 target cells with cell-free vector virus produced by transient cotransfection, together with the pCenv-1 plasmid in 293T cells. The transduction rates are the means and standard variations of six experiments. (B) PFV Gag and Pol proteins in cellular lysates and extracellular particles centrifuged through a sucrose cushion. 293T cells were transfected as indicated, and the viral proteins were analyzed by immunoblot. M, relative molecular mass standards in kilodaltons.