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Packaging of RNA from the pCgp-9 packaging constructs into PFV vector particles. 293T cells were transfected with the plasmids as indicated. Viral particles were prepared from the cell-free supernatant by centrifugation through sucrose, and the particle-associated RNA was analyzed by RPA by using the radiolabeled LTR-spanning pSP/HFV-2 probe (21).
