Comparison of genome organization between HCRSV (this study), CarMV (9), and CPMoV (42). Open rectangles, predicted ORFs in each viral genome; shaded rectangles, novel ORFs. The potential readthrough and in-frame UAG amber termination codons for the first ORF of each virus and the locations of various HCRSV ORFs, the full-length gRNA, and sgRNA1 and 2 are shown. Numbers represent nucleotide positions.