FIG. 6.

Analysis of the in vitro translation products from total viral RNA (lane 1) and gel-purified gRNA (lane 2) in wheat germ extracts separated on SDS–12% polyacrylamide gel. The radiolabeled in vitro translation products from total RNA were immunoprecipitated with either preimmune serum (lane 3) or anti-HCRSV (lane 4), separated on SDS–12.5% polyacrylamide gels, and visualized by autoradiography. The coat protein was also detected by electrophoresis of the purified virus particles on SDS–12.5% polyacrylamide gel and visualized by staining with Coomassie brilliant blue (lane 5). Numbers indicate molecular markers in kilodaltons (lane 6).