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. 2023 Apr 14;3:1168650. doi: 10.3389/fopht.2023.1168650

Figure 2.

Figure 2

Effect of alpha smooth muscle actin (αSMA) expression after TGFβ stimulation with and without miR-494 inhibition in human Müller cell line and human RPE cell lines. (A) Quantification of αSMA in human RPE and Müller cell lines by immunoblot (representative blots above) with and without 72h TGFβ treatment. (B) Representative immunofluorescent images of αSMA (red) in human RPE and Müller cell lines with and without 72h TGFβ treatment and counterstained with the nuclear dye 4′,6-diamidino-2-phenylindole (DAPI, blue). (C) Quantification of αSMA in human RPE and Müller cell lines by immunoblot (representative blots above) after 72h TGFβ treatment with and without pre-treatment and co-incubation with miR-494-3p inhibitor. (D) Representative immunofluorescent images of αSMA (red) in human RPE and Müller cell lines counterstained with the nuclear dye DAPI (blue) after 72h TGFβ treatment with and without pre-treatment and co-incubation with miR-494-3p inhibitor. Mean ± SEM, * p<0.05 TGFβ versus control condition.