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. 2023 Apr 14;3:1168650. doi: 10.3389/fopht.2023.1168650

Figure 4.

Figure 4

Effect of co-treatment with p27 siRNA and miR-494 inhibitor on TGFβ-induced αSMA expression. (A) Quantification of αSMA in RPE cells by immunoblot (representative blots above) after 72h TGFβ treatment with and without pre-treatment and co-incubation with miR-494 inhibitor with or without p27 siRNA co-transfection. (B) Representative immunofluorescent images of αSMA (red) in RPE cells counterstained with DAPI (blue) after 72h TGFβ treatment pre-treatment and co-incubation with miR-494 inhibitor with or without p27 siRNA co-transfection. (C) Quantification of αSMA in Müller cells by immunoblot (representative blots above) after 72h TGFβ treatment with and without pre-treatment and co-incubation with miR-494 inhibitor with or without p27 siRNA co-transfection. (D) Representative immunofluorescent images of αSMA (red) in Müller cells counterstained with DAPI (blue) after 72h TGFβ treatment pre-treatment and co-incubation with miR-494 inhibitor with or without p27 siRNA co-transfection. Mean ± SEM, * p<0.05 versus control condition. # p<0.05 between select conditions.