FIG. 3.

GFP expression of CCR5-expressing GHOST cells infected with HIV-1 clone ACH142-8G9, ACH142-32D2, ACH142-*E11, NL4-3, or JR-CSF. GHOST cells were infected with 0.5 ml of viral stocks in the presence of Polybrene (4 μg/ml). Forty-eight hours after infection, cells were harvested and fixed, and GFP expression was measured by flow cytometry with a FACScan.