TABLE 3.

Amino acid changes predicted to have accompanied E protein evolution of DEN^a

Period of evolution	Amino acid position	Change
DEN-1 emergence	6	V→I
	120	E→K
	345	I→V
DEN-2 emergence	122	L→K
	181	I→V
	236	M→T
	345	K→R
	379	V→I
DEN-4 emergence	19	T→A
	148	A→T
	203	G→K
	340	K→R
	342	M→V
	355	I→T
	364	I→V
	382	A→V
Sylvatic DEN-1 evolution	16	S→L
	157	E→G
Sylvatic DEN-2 evolution	59	Y→F
	113	I→V
	203	N→K
	331	S→A
	367	V→I
	446	A→V
	481	L→I
Sylvatic DEN-4 evolution	132	I→V
	154	D→S
	162	A→T
	478	T→S

^aUnique synapomorphies (shared and derived amino acids) represented in the branches designated E and S in Fig. 1.