FIG. 4.

Polymerization activities on a DNA template catalyzed by RSV RTs selectively mutated in the polymerase active site. Reactions were performed for 10 min at 37°C in RT buffer with 10 nM M13 substrate and enzyme and a 250 μM concentration of each dNTP (37). Lane M shows DNA size markers (their sizes are indicated on the left), and lane -RT shows P-T without enzyme.