FIG. 5.

DiIC₁₆(3) colocalizes with HIV, while DiIC₁₂(3) does not. Infected Jurkat cells were incubated with DiIC₁₆(3) (A) or DiIC₁₂(3) (B) for 15 min on ice before being fixed in 2% paraformaldehyde-PBS. Cells were then labeled with CD4Ig or anti-HIV IgG and FITC-labeled sheep anti-human IgG. Dialkylindocarbocyanine lipids are shown in red, FITC staining is shown in green, and the overlap is indicated in yellow. All of the micrographs in this figure were labeled with CD4Ig, except the last micrograph in panel A, which was labeled with anti-HIV IgG.