FIG. 7.

[³H]myristic acid-labeled Gag partitions to GEM domains. Jurkat cells labeled with [³H]myristic acid were treated with 1% Triton X-100 at 4°C and then equilibrium centrifuged. GEM domain fractions 3 to 5 were pooled and precipitated for Gag. IgG was used as a negative control. Soluble fractions 8 to 10 were pooled and precipitated for Gag as well. Precipitated proteins were blotted onto nitrocellulose and exposed to film. Blots were quantitated as described in Materials and Methods and expressed as a percentage of the total.