Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 May 7;43(12):2424–2452. doi: 10.1038/s44318-024-00104-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

PMC Copyright notice

Figure EV5 — (A) HeLa cells were transfected with control siRNA or Sgo1 siRNA. At 28 h post-transfection, cells were arrested in S-phase with thymidine treatment for 20 h, and then released into fresh medium. At 9 h post-release, cells were treated for 2 h with nocodazole, then mitotic cells were collected and then cytospun onto coverslips, fixed, and immunostained with antibodies for Sgo1, H2ApT120 and CENP-C, and DAPI. Example images are shown. White arrows point to Sgo1 distributed on chromosome arms. The yellow arrow points to Sgo1 enriched at mitotic centromeres. (B) HeLa cells were transfected with control siRNA, CENP-U siRNA, and/or Sgo1 siRNA. At 48h post-transfection, cells were treated with nocodazole for 3 h, then mitotic cells were collected to prepare chromosome spreads, and then stained with the CENP-C antibody and DAPI. The percentage of cells in which the majority of sister chromatids was separated or unseparated was determined in 300 cells for each condition from three independent experiments, with statistics being analyzed for cells with separated chromatids. (C) Asynchronous cells were fixed and immunostained with antibodies for Sgo1, H2ApT120, CENP-C, and DAPI. Example images for cells at the indicated stages of the cell cycle are shown. (D, E) Control HeLa cells and Sgo1-K492A mutant cells were transfected with control siRNA or CENP-U siRNA. At 48 h post-transfection, cells were subjected to nocodazole treatment for 3 h. Mitotic chromosome spreads were stained with the CENP-C antibody and DAPI. The inter-KT distance was measured on over 1000 chromosomes in 20 cells. Data from two individual experiments are shown (D). Example images are shown (E). Related to Fig. 8C. (F) Multiple sequence alignment for the Y/F-x-F motif-containing region of Sgo1 in the indicated vertebrates. Data information: Statistics were performed using unpaired Student’s t-test (B, D). Means and SDs are shown (B, D). Scale bars, 10 µm (A, C, E).