Fig. 5.

GSK126 inhibits proliferation and H3K27me3 of OS cells. a OS cell lines were treated for 48 h with GSK126, and their proliferation was assessed using the WST-1 assay. GSK126 inhibited the proliferation of KRIB, HOS, and SJSA-1 cells. Half-maximal inhibitory concentrations (IC₅₀) values are available in Table S4. b and c Treatment with GSK126 induced apoptosis and cell-cycle arrest in OS cells. b Results of annexin V–fluorescein isothiocyanate/propidium iodide analysis of the indicated OS cell lines after 24 h of treatment with GSK126 (P < .01). The error bars represent standard error of the mean. c, Results of flow cytometric analysis of the cell-cycle progression in OS cell lines after 24 h of treatment with GSK126. GSK126 induced sub-G1 arrest in KRIB, SJSA1, and CCH-OS-D cells and S-phase arrest in HOS cells (P < .05). The error bars represent standard error of the mean **P < 0.01; ***P < 0.001; ****P < 0.0001. d Evaluation of H3K27me3 in SJSA1, KRIB and CCH-OS-D cell lines following GSK126 treatment for 72 h. Total histone H3 is shown as a loading control