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Construction and functional expression of VP22-GFP fusions. HVP22 and BVP22 gene homologs were amplified by PCR and subcloned into a GFP variant-encoding mammalian expression vector. (A) Nucleic acid and amino acid changes to the wild-type VP22 homolog (boldface) through the start codon for the fused GFP. (B) Western immunoblot of expressed protein from these constructs utilizing anti-GFP.
