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Nuclear membrane lysis of VP22 homolog-transfected D17 cells accentuates differences in nuclear protein fraction labeling. BVP22-transfected cultures have a halo appearance, whereas HVP22-transfected cultures have accentuated nucleolus labeling. Lysis of VP22-expressing cells (arrows; upper panels) resulted in nuclear labeling of the entire monolayer for both homologs. Scale bar, 2 μm.
