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. 2024 Apr 30;43(12):2506–2525. doi: 10.1038/s44318-024-00102-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV3 — (A) Flow-cytometric analyses of the indicated B cell types in the bone marrow of Cd79a-Cre R26^LSL-HenT6B/+, Cd79a-Cre R26^{LSL-HenT6B/LSL-HenT6B} and control R26^{LSL-HenT6B/LSL-HenT6B} mice at the age of 6–8 weeks. The percentage of cells in the indicated gates is shown. One of three independent experiments is shown. (B) Flow-cytometric analysis of B and T cells in the spleen of Cd79a-Cre R26^LSL-HenT6B/+, Cd79a-Cre R26^{LSL-HenT6B/LSL-HenT6B} and control R26^LSL-HenT6B/+ or R26^{LSL-HenT6B/LSL-HenT6B} mice at the age of 6–8 weeks. The percentage of B and T cells is shown next to the respective gate (left). The GFP expression of B and T cell of the indicated genotypes is shown as a histogram (right). One of three independent experiments is shown. (C) Total RNA was extracted from B cells of the indicated genotypes (see Methods for purification strategy), mixed with four methylated and four unmethylated RNA spike-ins and subjected to mime-seq. Libraries were normalized to methylated spike-ins. Normalized reads of oxidized vs. unoxidized libraries are plotted for every miRNA with > 0.5 norm. reads. Note the depletion of unmethylated miRNAs (similar to the four unmethylated spike-ins) and the strong enrichment when HENMT1^∆C-T6B is expressed (close to the four methylated spike-ins).