Reagents and tools table
| Reagent/resource | Reference or source | Identifier or catalog number |
|---|---|---|
| Experimental models | ||
| HEK293T cell line (H. sapiens) | Takara | 632180 |
| RKO cells (H. sapiens) | ATCC | CRL-2577 |
| MEF cells (M. musculus) | Laboratory of Gijs Versteeg, University of Vienna | N/A |
| Mouse: R26(LSL-miR17-92) | Laboratory of Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine (Xiao et al, 2008) | Stock#008517; RRID: IMSR_JAX:008517 |
| Mouse: Bhlha15-Cre | Laboratory of Stephen F. Koniecszny, Purdue University | N/A |
| Mouse: Cd79a-Cre | The Jackson Laboratory | Stock#020505, RRID: IMSR_JAX:020505 |
| Recombinant DNA | ||
| Plasmids | Table EV1 | |
| Antibodies | ||
| Mouse anti-myc | Merck Millipore | 05-724 |
| Rabbit anti-At-HEN1 | Agrisera | AS15 3095 |
| Rabbit anti-actin | Sigma | A2066 |
| anti-rabbit HRP | Cell Signaling Technology | 7074 |
| anti-mouse HRP | Cell Signaling Technology | 7076 |
| anti-FLAG | Sigma | F1804 |
| Kit-PeCy7 (ACK) | Biolegend | b135112 |
| Kit-PeCy7 (2B8) | Invitrogen | 25-1171-82 |
| CD25-PE (PC61.5) | BD Bioscience | 553866 |
| IgM-PerCP-eFlour710 (II/41) | Invitrogen | 46-5790-82 |
| B220-BV785 (RA3-6B2) | BD Bioscience | 563894 |
| IgD-APC (11-26C) | Invitrogen | 17-5993-82 |
| CD4-BV510 (GK1.5) | BD Bioscience | 569249 |
| CD8a-BV605 (53-67) | BD Bioscience | 563152 |
| CD21/CD35-PE (7G6) | BD Bioscience | 562756 |
| CD19-BV421 (1D3) | BD Bioscience | 562701 |
| TCRb-APC-eFlour780 (H57-597) | Invitrogen | 47-5961-80 |
| CD138-APC (281-2) | BD Bioscience | 561705 |
| TACI-PE (8F10) | BioLegend | 133403 |
| Oligonucleotides and sequence-based reagents | ||
| DNA/RNA oligo sequences | Table EV1 | |
| Chemicals, enzymes, and other reagents | ||
| Gibson Assembly Master Mix | New England Biolabs | E2611L |
| DMEM | Corning | 10-013-CV |
| RPMI | Corning | 10-040-CV |
| FBS | Avantor | 89510186 |
| l-glutamine | Gibco | 25030081 |
| Sodium Pyruvate | Sigma | S8636 |
| HEPES | Sigma | H4034-500G |
| Polyethylenimine, Linear, MW 25000 | Polysciences | 23966 |
| Doxycycline | Sigma | D9891-1G |
| Opti-MEM | Gibco | 31985062 |
| PBS | Corning | 21-040-CV |
| Trizol | Invitrogen | 15596026 |
| Polybrene | Sigma | TR-1003-G |
| 0.45 μm filters | Sarstedt | 83.1826 |
| Blasticidin | InvivoGen | ant-bl-05 |
| CD43-MicroBeads | Miltenyi Biotec | #130-049-801 |
| Pierce BCA Protein Assay | Thermo Scientific | 23227 |
| 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels | BioRad | 4561093 |
| Nitrocellulose membrane | BioRad | |
| Clarity Western ECL | BioRad | 1705060 |
| 2-propanol | Fisher Chemical | A416-1 |
| Chloroform | Fisher Chemical | C298-500 |
| Glycogen | Sigma | G1767-1VL |
| EtOH abs. | Avantor | 20821.321 |
| H2O, DEPC-treated | Invitrogen | AM9920 |
| H2O, Rnase free | Invitrogen | AM9935 |
| Qubit RNA BR Assay Kit | Invitrogen | Q10210 |
| Ammonium Persulfate | Sigma | A3678 |
| TEMED | Sigma | T9281 |
| Sodium Periodate | Sigma | 311448-5G |
| Sodium Acetate (3 M) ph 5.5 | Invitrogen | AM9740 |
| NaOH | Sigma | S5881 |
| Boric acid | Sigma | B6768 |
| Sodium tetraborate | Sigma | 311448-5G |
| Sodium chloride | Sigma | 106404 |
| SequaGel UreaGel System | National Diagnostics | EC-833 |
| Gel Loading Buffer II | Invitrogen | AM8547 |
| Hybond-NX membrane | Cytiva | RPN303T |
| Methylimidazole | Sigma | M50834 |
| EDC | Sigma | E7750-25G |
| EDTA (0.5 M) pH 8 | Invitrogen | 15575020 |
| Sodium Phosphate dibasic heptahydrate | Sigma | 431478 |
| Phosphoric Acid | Avantor | 02-003-602 |
| T4 Polynucleotide Kinase | New England Biolabs | M0201L |
| γ-32P-ATP | Perkin Elmer | BLU002Z250UC |
| MicroSpin G-25 Columns | Cytiva | 27532501 |
| Storage Phosphor Screen | Cytiva | 28956474 |
| Protein G Dynabeads | Invitrogen | 10003D |
| K227Q truncated T4 RNA ligase 2 | New England Biolabs | M0351L |
| PEG8000 50% | New England Biolabs | B1004A |
| SYBR Gold | Invitrogen | S11494 |
| TBE buffer, 10x | Quality Biological | 351-001-131 |
| SSC buffer, 20x | Sigma | S6639-1L |
| T4 RNA ligase 1 | New England Biolabs | M0204L |
| Superscript II or III reverse transcriptase | Invitrogen | 18064014/18080044 |
| RNaseOUT | Invitrogen | 10777019 |
| ExoSAP-IT | Applied Biosystems | 78201 |
| Kapa HiFi HotStart Library Amp kit | Roche | KK2612 |
| Zymoclean Gel DNA recovery kit | Zymo | D4008 |
| Low Range Agarose | BioRad | 1613107 |
| IGEPAL CA-630 | Sigma | I3021 |
| Sodium Deoxycholate | Sigma | D6750 |
| Sodium Dodecyl Sulfate | Sigma | L3771 |
| Sodium Dodecyl Sulfate 20% solution | Sigma | 05030 |
| Tris | Thermo Scientific | 75825 |
| Glycerol | Sigma | 15523 |
| Bromophenol Blue | BioRad | 1610404 |
| 2-Mercaptoethanol (BME) | Sigma | M-6250 |
| Tween | Sigma | P9416 |
| Magnesium Chloride | Avantor | 7791-18-6 |
| Software | ||
| FlowJo 10.10 (Treestar) | https://www.flowjo.com | |
| GraphPad Prism 10.1.1 | https://www.graphpad.com | |
| R Studio v2022.12.0 | https://posit.co | |
| Adobe Illustrator v25.4.1 | https://www.adobe.com | |
| Other | ||
| LSRFortessa | BD Biosciences | |
| FACSAria III | BD Biosciences | |
| Aurora | Cytek | |
| Trans-Blot SD | BioRad | |
| Typhoon phosphorimager | Cytiva | |
| HiSeq V4 | Illumina | |
| NovaSeq | Illumina | |
| Qubit flex fluorometer | Invitrogen | |
| Blue light transilluminator | ||
| ChemiDoc | BioRad | |