Fig. 2.
Surgical denervation and antagonism of trigeminal A2AR with SCH58261 inhibited OSCC growth. a Tumor volume of HSC3 xenografts in mice undergoing sham surgery or lingual nerve denervation, n = 4 mice (Left panel). Representative HE images of HSC3 xenograft (circled) in mice undergoing sham surgery or lingual denervation (Right panel). Scale bar, 500 µm. b Immunofluorescent staining of neurofilament light chain (NFL, arrowheads) in framed area of a. Scale bars, 100 µm. c Relative mRNA expression of Adora2a in trigeminal ganglia of healthy mice or mice carrying HSC3 tumor xenograft, n = 3 mice. d Phosphorylation of CREB and total CREB in trigeminal ganglia of healthy mice, mice carrying HSC3 tumor xenograft, and tumor-bearing mice treated with 5 mg/kg SCH58261 for 3 h. e In vivo growth of HSC3 xenograft in nontreated mice and those treated with 5 mg/kg SCH58261, n = 6 mice. (Left panel) Representative HE images of HSC3 xenografts in mice treated with vehicle or 5 mg/kg SCH58261 (Right panel). Scale bar, 1 mm. Statistical analysis was conducted using unpaired Student’s t-test (a, c, e)