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Immune markers detected in sacculus rotundus by immunohistochemistry.
| Antigen (clone) | Host | Specificity and target cells | Dilution | |
|---|---|---|---|---|
| CD163 (anti-human) | Mouse | Expressed in M2 macrophages | 1:300 | |
| MAC387 (anti-human) | Mouse | Expressed in activated and recently recruited macrophages | 1:200 | |
| IFNγ (anti-bovine) | Mouse | Induces M1 macrophage polarization | 1:100 | |
| TNFα (anti-bovine) | Mouse | Expressed in M1 macrophages | 1:250 | |
