FIG. 3.

Micrographs of readministration of ALP staining in the broncholes of rhesus monkeys. Rhesus monkeys were administered Ad-ALP in the right lower lobe as depicted in Fig. 1 top. Animals were necropsied, and the right lower lobe was sectioned for ALP expression, as described in Materials and Methods. Alkaline phosphatase expression in the broncholes of animals T7N (A) and T8P (B) were analyzed by staining with buffer containing BCIP and NBT, as described in Materials and Methods. There was significant alveolar endogenous ALP staining in all the tissue. However, extensive analyses of lungs of rhesus monkeys not administered ALP-expressing adenovirus showed that none of the broncholar epithelial cells had endogenous ALP staining. Panel A, animal T7N; panel B, animal T8P; magnification, ×186.