FIG. 4.

hu5C8 treatment inhibits generation on inflammatory cytokine IL-8 in the lung. Rhesus monkeys were instilled with Ad-CFTR on day 1 with or without hu5C8 as described in the legend to Fig. 1 bottom. BAL obtained on day −2 (prior to vector instillation) and 29 were analyzed for the presence of IL-8 by ELISA. Treatments of the animals in the various groups are described in the legend to Fig. 1 bottom. All of the 5C8-treated animals had a significant decrease in induction of IL-8 (P < 0.05, Wilcoxon's test) as compared to those who received vector but were not administered 5C8. Since the rhesus monkeys were caught in the wild, the prevector inflammatory status of the lung could not be controlled. All the monkeys underwent a 3-month quarantine period, when they were tested for a variety of infectious agents; all tests were negative.