FIG. 2.

sH3L protein bound to cell surface HS. BSC40 (A to C), L (D), gro2C (E), or sog9 (F) cells were incubated with PBS (black line) or biotinylated H3L (red line) and analyzed with a FACS as described previously (22). Alternatively, as shown in panels A to C, biotinylated H3L protein was mixed with 10 μg/ml (results shown by green line) or with 100 μg/ml (results shown by blue line) of soluble GAGs and analyzed with a FACS. The soluble GAGs used in competitions were HS (A), CS (B), or DS (C).