Table 3.

Intra-reproducibility and intra-repeatability of the MGF_110-1L gene and O61R gene by the duplex fluorescent quantitative PCR.

Target gene	Template concentration (copies /μL)	Intra-assay variation			Intra-assay variation
		Average value	Standard deviation	CV	Average value	Standard deviation	CV
MGF_110-1L	2.95 × 105	17.73	0.09	0.51%	17.91	0.07	0.39%
	2.95 × 104	20.74	0.05	0.24%	21.49	0.08	0.37%
	2.95 × 103	24.72	0.08	0.32%	24.63	0.05	0.20%
	2.95 × 102	28.06	0.13	0.46%	28.29	0.11	0.39%
	2.95 × 101	31.13	0.13	0.42%	31.66	0.09	0.28%
O61R	2.95 × 105	18.72	0.07	0.37%	18.66	0.06	0.32%
	2.95 × 104	21.89	0.09	0.41%	22.36	0.03	0.13%
	2.95 × 103	25.89	0.06	0.23%	25.63	0.03	0.12%
	2.95 × 102	29.21	0.08	0.27%	29.35	0.09	0.31%
	2.95 × 101	32.30	0.11	0.34%	32.82	0.13	0.40%