FIGURE 2.

Effect of GIP on the ROS level in neurons under oxidative stress. (A, B) GIP decreased ROS level after H₂O₂ treatment. ROS signals were probed by DCFH‐DA. Scale Bar = 200 μm. n = 100–150 neurons for each group per test, N = 3 (cells were from rats interpedently). The data were shown as mean ± SE, and were analyzed by one‐way ANOVA, ****p < 0.0001. (C, D) GIP decreased the number of apoptotic cells. The cell apoptosis was determined by TUNEL assay. n = 30 for each group per test, N = 3. The data were shown as mean ± SE, and were analyzed by one‐way ANOVO, ***p < 0.001, ****p < 0.0001. Scale Bar = 200 μm. (E, F) GIP increased the ratio of BCL2/BAX protein after treatment of H₂O₂. The data were normalized by negative ctrl (NC) group. n = 3. The data were shown as mean ± SE, and were analyzed by one‐way ANOVA, **p < 0.01, ****p < 0.0001.