Binding constant data (Kb, Ksv) along with % hypochromism (% H) obtained from the interaction study of the compounds with ctDNA and tRNA.
Compd | λ max | Absorption spectroscopy | Emission spectroscopy (at λem = 580 nm) | ||||||
---|---|---|---|---|---|---|---|---|---|
(Kb), × 106 | % H* | (Ksv),×104 | % H | ||||||
ctDNA | tRNA | ctDNA | tRNA | ctDNA | tRNA | ctDNA | tRNA | ||
HL | 314 | 1.10 | 1.36 | 8.35 | 7.20 | 0.34 | 1.19 | 32.4 | 26.1 |
(C1) | 320 | 4.71 | 10.3 | 12.7 | 8.67 | 4.91 | 7.52 | 55.4 | 61.5 |
(C2) | 329 | 2.58 | 8.96 | 10.4 | 7.60 | 1.42 | 4.51 | 54.2 | 48.8 |
(C3) | 319 | 1.22 | 1.51 | 6.81 | 6.66 | 0.52 | 1.6 | 50.1 | 26.9 |