FIG. 4.

Residues S₃ and L₁₆ are important for the binding of p10 to p24 P. Interaction of GST-p24 (2.5 μg; 500 μM) with FLAG-tagged p10 deletion mutation proteins (0.5 μg; 500 μM) bound to solid phase was performed as described in the legend to Fig. 2. The bound proteins were resolved by denaturing SDS–15% polyacrylamide gel electrophoresis and electrotransferred onto a nitrocellulose membrane, and Western blotting was performed with anti-p24 antiserum (top blot) or with the anti-FLAG monoclonal antibody (bottom blot). GST-p24 interacted with the peptide 1-17-FLAG (lane 1), p10Δ2-FLAG (lane 3), and the peptide 1-16-FLAG (lane 6), but not with p10Δ17-FLAG (lane 2), p10Δ3-FLAG (lane 4), or the peptide 1-15-FLAG (lane 5).