Figure 3.

Microarray reactivity analysis of sera from mice immunized with CRM197 and PA63 glycoconjugates toward oligosaccharides. (a) Pooled response of DECA-CRM197 and DECA-PA63 mice. (b) A heatmap of comparing glycan binding profiles of sera from both conjugates. Rows A–C are DECA-PA63 mice, and rows D–F are DECA-CRM197 mice. The heatmap is normalized by lowest and highest point in each data set. The full library of the oligosaccharides structure can be found in Figure 4. A data point is the RFU of a single glycan spot on the array.