KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-CTLA-4 | BioSB | Cat#BSB2884; RRID: AB_2762365 |
| Rabbit monoclonal anti-PD-L1 | Cell Signaling Technology | Cat#13684; RRID: AB_2687655 |
| Mouse monoclonal anti-CD8 | Dako | Cat# M7103; RRID: AB_2075537 |
| Rabbit monoclonal anti-CD163 | BioSB | Cat#BSB3276 |
| Mouse monoclonal anti-CD68 | Dako | Cat#M0876; RRID: AB_2074844 |
| Rabbit monoclonal anti-LAG-3 | BioSB | Cat#BSB3366 |
| Mouse monoclonal ant-PanCK | Dako | Cat#M3515; RRID: AB_2132885 |
| Rabbit monoclonal anti-CD4 | Cell Marque | Cat#104R-15; RRID: AB_1516770 |
| LAG-3 | BioSB | Cat# BSB3366; RRID: AB-3073657 |
| CD163 | Cell Marque | Cat# AC-0316; RRID: AB_3073656 |
| Biological samples | ||
| Untreated metastatic castration-sensitive needle core biopsies: Fresh Samples | Columbia University Irving Medical Center | N/A |
| On-treatment (ADT or ADT + a-PD-1) metastatic castration-sensitive needle core biopsies: Fresh Samples | Columbia University Irving Medical Center | N/A |
| Untreated metastatic castration-sensitive needle core biopsies: FFPE tissue | Columbia University Irving Medical Center | N/A |
| On-treatment (ADT or ADT + a-PD-1) metastatic castration-sensitive needle core biopsies: FFPE tissue | Columbia University Irving Medical Center | N/a |
| Critical commercial assays | ||
| 10X Genomics Chromium Single Cell 5’ Library & Gel Bead Kit | 10X Genomics | N/A |
| Illumina NovaSeq 6000 Sequencing System | Illumina | N/A |
| Opal-7-color multiplex IHC kit | Akoya Biosciences | SKU NEL811001KT |
| Deposited data | ||
| Raw and Analyzed data used to generate analyses shown in this Manuscript: scRNASeq, bulkRNASeq, qmIF | This manuscript | Available on Mendeley Data https://doi.org/10.17632/5nnw8xrh5m.1 |
| Software and algorithms | ||
| HALO Image Analysis Software | Indica Labs | http://www.indicalab.com/halo/ |
| Seurat v3 | Stuart et al., 201983 | https://cran.r-project.org/web/packages/Seurat/index.html |
| SingleR | Aran et al., 201930 | https://bioconductor.org/packages/release/bioc/html/SingleR.html |
| InferCNV | (Tickle et al., 2019)84 | https://github.com/broadinstitute/infercnv |
| ARACNe | Lachmann et al., 201685 | https://github.com/califano-lab/ARACNe-AP |
| VIPER | Alvarez et al., 201627 | http://bioconductor.org/packages/release/bioc/html/viper.html |
| R Code Used to Generate Figures and perform analyses shown in this manuscript | This manuscript | Available on Mendeley Data https://doi.org/10.17632/5nnw8xrh5m.1 |