Table 1.
Summary of eIF4E partner proteins.
| Interactor | Localization | Techniques | Cell Line -Tissue | Year of publication | PMID | |
|---|---|---|---|---|---|---|
| 1 | ACVRL1/HHT | In vitro assay | Reconstituted Complex - Affinity Capture-Western | THP1 | 2015 | 25915158 |
| 2 | ASLX1 | Total Extract | AP-MS | HEK293T | 2022 | 36180891 |
| 3 | ASLX2 | Total Extract | AP-MS | HEK293T | 2022 | 36180891 |
| 4 | ASLX3 | Total Extract | AP-MS | HEK293T | 2022 | 36180891 |
| 5 | BCD | In vitro assay | Pull-Down (m7GTP-agarose) - Pull-Down | Drosophilia embryos | 2002 | 12368268 |
| 6 | BIRC2/cIAP1 | Total Extract | Coimmunoprecipitation - Pull Down | HEK293T | 2017 | 28852129 |
| 7 | CEFL1 | Total Extract | BioID | HEK293 | 2018 | 29395067 |
| 8 | CPEB | Total Extract | Pull-Down | oocyte extract | 1999 | 10635326 |
| 9 | CPSF1 | Nucleus | Coimmunoprecipitation | U2OS | 2019 | 31042468 |
| 10 | CPSF3 | In vitro assay | Coimmunoprecipitation | U2OS | 2019 | 31042468 |
| 11 | CRM1/XPO1 | Nucleus - In vitro assay | Coimmunoprecipitation - size exclusion chormatography | U2OS | 2009 - 2017 | 19262567 - 28325843 |
| 12 | CTNNB1/ß-catenin | Total Extract | Pull-Down (m7GTP-agarose) | A10 - HEK293T | 2018 | 30361391 |
| 13 | CYFIP1 | Total Extract | Pull-Down (m7GTP-agarose) - Coimmunoprecipitation | Total mouse brain - primary neurons | 2008 | 18805096 |
| 14 | DCC | Cytoplasm | Co-fractionation - Coimmunoprecipitation | Commissural neuron | 2010 | 33930296 |
| 15 | DDX3 | Nucleus | Coimmunoprecipitation | U2OS | 2009 | 19262567 |
| 16 | eIF3A | Total Extract | Coimmunoprecipitation | MCF7 | 2019 | 30573685 |
| 17 | eIF3B | Total Extract | Coimmunoprecipitation | 293T - MCF7 - HEK293E | 2006 - 2008 - 2019 | 16541103 - 18423201 - 30573685 |
| 18 | eIF3F | Total Extract | Coimmunoprecipitation | MCF7 | 2019 | 30573685 |
| 19 | eIF4A1 | Total Extract | Pull Down - Coimmunoprecipitation | MCF10A - CRL2324 - HTB20 - KT1 | 2006 - 2012 | 16648488 |
| 20 | eIF4A2 | Total Extract | BioID | HEK293 | 2018 | 29395067 |
| 21 | eIF4EBP1 | Total Extract | Coimmunoprecipitation - Pull Down- crystallography- NMR | Sf9 | 1994- 1995 | 7935836 - 7651417 |
| 22 | eIF4EBP2 | Total Extract | Pull Down - Crystallography | HEK293 - HEK293E | 1994 - 2010 | 7935836 - 20347422 |
| 23 | eIF4EBP3 | Total Extract | Coimmunoprecipitation - Pull-Down | HeLa | 1998 | 9593750 |
| 24 | eIF4EBP3 | Cytoplasm | Coimmunoprecipitation | T cells - HEK293 - HeLa | 2002 | 12482586 |
| 25 | eIF4ENIF1/4E-T | Total Extract | Coimmunoprecipitation - Far western | HeLa | 2000 | 10856257 |
| 26 | eIF4G | Total Extract - Cytoplasm - In vitro assay | Purification of eIF4F complex - Pull Down - Coimmunoprecipitation - NMR - Crystallography | Rabbit reticulocyte - Sf9 - U2OS | 1983 - 1995 - 2009 -2016 | 6853548 - 8521827 - 19262567 - 27773676 |
| 27 | eIF4G2 | Total Extract | Coimmunoprecipitation | BY4741 | 2023 | 37449412 |
| 28 | eIF4G3 | Total Extract | Coimmunoprecipitation - Far western | HeLa | 1998 | 9418880 |
| 29 | ELAVL1/HuR | Nucleus | Coimmunoprecipitation | U2OS | 2023 | 36843541 |
| 30 | Emx2 | Cytoplasm | Coimmunoprecipitation | COS-7 | 2004 | 15247416 |
| 31 | Engrailed 2 | Cytoplasm | Coimmunoprecipitation | COS-7 | 2004 | 15247416 |
| 32 | Fi(2)d | Nucleus | Coimmunoprecipitation | Drosophila embryo | 2011 | 21829374 |
| 33 | FMR1 | Total Extract | Pull-Down (m7GTP-agarose) | A10 - HEK293T | 2018 | 30361391 |
| 34 | G3BP1 | Total Extract | BioID | HEK293 | 2018 | 29395067 |
| 35 | GEMIN5 | In vitro assay | Pull-Down | - | 2006 | 16739988 |
| 36 | hnRNPA1 | Nucleus | Coimmunoprecipitation | U2OS | 2009 | 19262567 |
| 37 | HOX11 | In vitro assay | pulldown - NMR | - | 2003 | 12554669 |
| 38 | HOXA9 | Cytoplasm - Nucleus - In vitro assay | Pull-Down - Coimmunoprecipitation-Circular Dichroism | U937 - leukemia patients | 2005 | 15657436 |
| 39 | HSPB2 | Total Extract | Coimmunoprecipitation | LNCaP | 2010 | 20101233 |
| 40 | Importin 8 | In vitro assay | Pull-Down - Coimmunoprecipitation - NMR | 2016 | 27114554 | |
| 41 | LRPPRC | Nucleus - In vitro assay | Coimmunoprecipitation - Colocalization - Pulldownm - NMR - Size exclusion chromatography | U2OS | 2009 - 2017 | 19262567 - 28325843 |
| 42 | Maskin | Total Extract | Pull-Down | oocyte extract | 1999 | 10635326 |
| 43 | METTL3 | Total Extract - Cytoplasm | Coimmunoprecipitation | HeLa - H1299 | 2018 | 30232453 |
| 44 | MNKNK1 | Total Extract | Coimmunoprecipitation | 293T | 1999 | 9878069 |
| 45 | mTOR | Total Extract | Coimmunoprecipitation | HEK293E | 2008 | 18423201 |
| 46 | NCBP1/CBP80 | Total Extract | Coimmunoprecipitation | HeLa | 2013 | 23665581 |
| 47 | OTUD6B | Total Extract | Coimmunoprecipitation | H1299 | 2017 | 27864334 |
| 48 | Otx2 | Cytoplasm | Coimmunoprecipitation | COS-7 | 2004 | 15247416 |
| 49 | PABPC1 | Total Extract | Coimmunoprecipitation - BioID | HeLa - HEK293 | 2013 - 2018 | 29395067 |
| 50 | PATL1 | Total Extract | BioID | HEK293 | 2018 | 29395067 |
| 51 | PHAS-I | Total Extract | Far western | - | 1994 | 7935836 |
| 52 | PML | Total Extract - In vitro assay - Nucleus | Co-localization - Pull-Down - NMR - Circular Dichroism - Reconstituted Complex - CoImmunoprecipitation | NIH3T3 - human 551 fibroblasts - U937 - K562 - HEK293T | 2000 - 2001 - 2002 | 10763819 - 11500381 - 12167712 |
| 53 | PRH/HHEX | Total Extract -Cytoplasm - Nucleus | Coimmunoprecipitation - Co-localization - PullDown- Ciruclar Dichroism | leukemia patients - U937 | 2003 - 2005 | 14645512 |
| 54 | PRPF19 | Nucleus | Coimmunoprecipitation | U2OS - NOMO | 2023 | 36843541 |
| 55 | PRPF31 | Nucleus | Coimmunoprecipitation | U2OS - NOMO | 2023 | 36843541 |
| 56 | PRPF6 | Nucleus | Coimmunoprecipitation | U2OS - NOMO | 2023 | 36843541 |
| 57 | PRPF8 | Nucleus | Coimmunoprecipitation | U2OS - NOMO | 2023 | 36843541 |
| 58 | RNF113A | Total Extract | Peptide Pull-Down | SCLC | 2022 | 35819319 |
| 59 | RNMT | Nucleus - In vitro assay | Coimmunoprecipitation - Pull-Down - NMR | U2OS | 2022 | 35026230 |
| 60 | RPS6KB1 | Total Extract | Coimmunoprecipitation | HEK293E | 2008 | 18423201 |
| 61 | SF3B1 | Nucleus | Coimmunoprecipitation | U2OS - NOMO | 2023 | 36843541 |
| 62 | Snf | Nucleus | Coimmunoprecipitation | Drosophila embryo | 2011 | 21829374 |
| 63 | SNRNP200 | Nucleus | Coimmunoprecipitation | U2OS - NOMO | 2023 | 36843541 |
| 64 | SRSF3 | Cytoplasm | Coimmunoprecipitation | HEK293T | 2016 | 27381497 |
| 65 | STUB1/CHIP | Total Extract | Coimmunoprecipitation - Pull-Down | HEK293T | 2006 - 2017 | 16720573 - 28852129 |
| 66 | Sxl1 | Nucleus | Coimmunoprecipitation | Drosophila embryo | 2011 | 21829374 |
| 67 | TRIM22 | Total Extract | Coimmunoprecipitation | 293T -17 | 2012 | 22509910 |
| 68 | U1-70K/SnRNP70 | Nucleus | Coimmunoprecipitation | Drosophila embryo | 2011 | 21829374 |
| 69 | U2AF1/U2AF35/U2AF38 | Nucleus | Coimmunoprecipitation | U2OS - Drosophila embryo | 2023 - 2011 | 36843541 - 21829374 |
| 70 | U2AF2 | Nucleus | Coimmunoprecipitation | U2OS - NOMO | 2023 | 36843541 |
| 71 | U2AF50/Mud2 | Nucleus | Coimmunoprecipitation | Drosophila embryo | 2011 | 21829374 |
| 72 | UAP56 | Nucleus | Coimmunoprecipitation | U2OS | 2009 | 19262567 |
| 73 | UPF1 | Total Extract | Coimmunoprecipitation | HeLa | 2013 | 23665581 |
| 74 | VPg | In vitro assay | NMR - ITC - Fluorescence assay- XLMS | U2OS | 2019 | 31712417 |
| 75 | YTHDF2 | Total Extract | BioID | HEK293 | 2018 | 29395067 |
| 76 | Z protein (Lassa and LCMV) | In vitro assay | Pull-Down - NMR - Circular Dichroism | - | 2001 - 2010 | 11575918 - 20212144 |
| 77 | ZFP36 | Total Extract | BioID | HEK293 | 2018 | 29395067 |
| 78 | ZRANB1 | Total Extract | Coimmunoprecipitation | HepG2 | 2022 | 35944360 |
This list is not exhaustive and thus the absence of factors here should not be interpreted as factors not binding. In many cases, there are multiple references for interactions, in these cases the earliest relevant references were used and, in some cases, multiple references supporting the original finding are also included.