Figure 4.
Complement C3 is colocalized with cells in various pulmonary sub compartments. (A) Colocalization of C3 (green) and SARS2-N501YMA30 nucleocapsid protein (red) on 2 and 4 dpi. n= 5, blue = DAPI, red = nucleocapsid protein, green = C3, white arrows indicate areas with C3 and nucleocapsid protein colocalization. (B) Staining for complement C3 on lung tissue from control (top row) and following infection with SARS2-N501YMA30 (bottom row) on 2 and 4 dpi. White arrows indicate areas of C3 staining. n = 5, green = C3, blue = DAPI. (C) Colocalization of C3 with alveolar type I cells, (D) ciliated airway cells, and (E) endothelial cells on 4 dpi. White arrows indicate regions of colocalization with C3. dpi = days post-infection, vWF = von Willebrand Factor.