Figure 3: Vasculogenic mimicry phenotype decreases cell area on stiff ECM, which is rescued by increasing pHi in metastatic lung carcinoma.

a) Representative images of H1299 cells plated on soft (0.5% PEGDA) and stiff (4% PEGDA) HA gels and stiff (4% PEGDA) with raised pHi. Images show differential interference contrast (DIC), Hoechst 33342 (DNA, cyan) and CellMask Deep Red membrane stain (Cy5, magenta). Scale bars: 50 μm. b) Quantification of single-cell area collected as shown in (a) (n=3 biological replicates, n=1061 soft, n=954 stiff, n=1078 stiff high pHi. Red lines show medians ± IQR).