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[Preprint]. 2024 Jun 4:2024.06.04.597454. [Version 1] doi: 10.1101/2024.06.04.597454

Figure 4: Increased pHi reduced β-catenin abundance and nuclear localization in stiff matrix conditions.

Figure 4:

a) Representative images of H1299 cells plated on soft (0.5% PEGDA), stiff (4% PEGDA) and stiff with raised pHi (4% PEGDA) HA gels fixed and stained for β-catenin. β-catenin is pseudocolored according to scale. Scale bars: 50 μm. b) Quantification of whole cell β-catenin intensity collected as shown in (a). (n=3 biological replicates, n=452 soft, n=486 stiff, n=415 stiff high pHi. Red lines show medians ± IQR). c) Quantification of nuclear β-catenin intensity collected as described in (a). (n=3 biological replicates, n=1043 soft, n=975 stiff, n=1157 stiff high. Red lines show medians ± IQR). For (b) and (c), significance was determined by a Kruskal-Wallis test (*P<0.05; **P<0.01; ***P<0. 001; ****P<0.0001).