| Primary Antibodies and Detection Methodology | |||
|---|---|---|---|
| Protein | Source | Dilution | Detection Method |
| Calbindin | Abcam Ab82812 | 1:300 | Invitrogen goat anti-mouse secondary antibody, Alexa Fluor 488 or goat anti-rabbit secondary antibody, Alexa Fluor 594 for double immunostaining |
| PACT/RAX | ProteinTech 10771–1-AP | 1:100 | Invitrogen goat anti-rabbit secondary antibody, biotin followe Invitrogen streptavidin, HRP, and DAB |
| p-eIF2α | CST #9721 | 1:100 | Invitrogen goat anti-rabbit secondary antibody, biotin followe Invitrogen streptavidin, Alexa Fluor 488 |
| CreP | Abcam Ab220949 | 1:200 | Invitrogen goat anti-rabbit secondary antibody, biotin followe Invitrogen streptavidin, Alexa Fluor 488 |
| ATF4 | CST #11815 | 1:500 | Invitrogen goat anti-rabbit secondary antibody, biotin followe Invitrogen streptavidin, Alexa Fluor 488 |
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