Figure 4. Non-failing CS-Mpc2−/− hearts from young mice display increased glycolysis, but no glycogen accumulation.

A through K, ¹³C % enrichment of glucose (A), gluocse-6-phosphate (G6P) (B), fructose bisphosphate (FBP) (C), dihydroxyacetone phosphate (DHAP) (D), 2/3-phosphoglycerate (PG) (E), phosphoenolpyruvate (PEP) (F), pyruvate (G), lactate (H), alanine (I), and uridine diphosphate (UDP)-glucose (J), and the UDP-glucose normalized pool size (K) in hearts of young non-failing CS-Mpc2−/− and fl/fl littermates injected i.p. with U-¹³C-glucose, n=8–10. L, Heart glycogen content measured biochemically in young non-failing CS-Mpc2−/− and fl/fl littermates, n=6–7. M, Representative transmission electron microscopy images at 15,000X magnification of the cardiac papillary muscle in young fl/fl and CS-Mpc2−/− littermates. Scale bars = 1 μm. Data are presented as the mean±SD, and were analyzed by unpaired, two-tailed Student’s t-test with Welch correction.